DNA Extraction – SCOPES-DF

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Author

Studio 5
Studio 5
Informal educator
In line with the Ministry of Communications and Information Technology’s Digital Youth Strategy, STUDIO 5 is mainly aimed at nurturing youth as digital learners by sharpening their 21st century learning skills, as they develop in an all-pervasive digital environment. In… Read More

Summary

On-Site Session aims towards delivering the knowledge of how art and science are connected together through science experiments.

The session will focus on how to extract the DNA from the strawberry in order to picture it under the microscope and create art out of it.

What You'll Need

We will need :

  1. Plastic bags
  2. 25ml Graduated cylinder
  3. Cheesecloth
  4. Centrifuge tube
  5. Eye droppers
  6. Alcohol
  7. Wood splints
  8. Non-Iodized salt
  9. Plastic funnel
  10. Dish soap
  11. strawberry
  12. Presentation.

 

The Instructions

Introduction

In this section we will introduce the participant to the program, and we will ice-break to motivate them.

1- The full program is a 3 days start with extracting the DNA and ends as an art.

2- We will explain the steps to the participant as we are going to start with extracting the DNA from the strawberry, and then we will assemble a microscope (Foldscope), then we will design the picture we will take from the microscope, and we will vinyl cut it.

3- We will then Ice-Break with the participant through a question.

4- Then we will explain the meaning of science for the participant, As a knowledge that study the physical world and phenomena.

5- Then we will describe art to the participant as any human activity that express creativeness also art can be a matter of human view as it differs from one to another.

6- Then we will start a linking between the science and the art through mentioning a common source of both, as physical world and phenomena are expressed and described from the nature around also, art started from the nature, and it will end there as the main source of beauty is nature.

7- Then we will ask them a question regarding the traits they have, how it came to them.

8- Then we will explain and describe that the DNA is in fact the reason of transferring the traits from the parents to the children’s.

9- Then we will show them a video explains the DNA.

10- Then we will ask them about the uses of the DNA.

What we will be doing today

In this section we will show the participant what we will be doing also we will describe the tools we will be using in order to have the DNA extracted.

1- We will explain what we will be doing.

2- Then we will show the tools we have to use in order to extract the DNA.

3- Now we will start with the participant step by step.

 

4- The first step is the (Detergent solution)

  • We can prepare this solution in advance (soap, salt, and water), never shake the container just move the stick very lightly.

5- The detergent solution is the one responsible to destroy the cellular wall of the cell, so the DNA can be accessible.

6- After preparing the Detergent solution, we need to put the strawberries inside the plastic bag and start smashing them.

7- Then we will pour the detergent solution on the smashed strawberries. (about 20 to 30 ml for each strawberry piece).